Silencing CGRP neurons in the parabrachial nucleus does not impair active avoidance learning and expands successful avoidance to high footshock intensities¶

Claim · All claims
Evidence · 1 study (2 entries) · causal r1–r2 · q3
Currency · newest study 2026 · not yet checked against its sources · not reviewed by a person

Subclaims¶

q3 i? TetTox mice displayed significantly higher avoidance success rates than controls at 0.4 and 0.5 mA, indicating CGRP silencing expanded the range of aversive intensities over which active avoidance could be learned and expressed. → Hanny Hayeon Lee 2026 q3 i? Retention test 3 success rate showed a significant CGRP manipulation × Footshock Intensity interaction. → Hanny Hayeon Lee 2026 (2)

Evidence¶

Hanny Hayeon Lee 2026¶

Hanny Hayeon Lee, Eul Han & Yong Sang Jo. (2026). CGRP inhibition in the PBN shifts defensive strategy from freezing to active avoidance in a platform-based active avoidance paradigm. npj Science of Learning. https://doi.org/10.1038/s41539-026-00453-3

q3 · i? · causal · r1

Experiment silencing PBN CGRP neurons via Cre-dependent TetTox in CalcaCre/+ mice (n = 7 per footshock intensity cohort) versus eYFP controls across 0.1-0.5 mA: "TetTox mice displayed signi ficantly higher avoidance success rates at 0.4 and 0.5 mA".

"Consistent with these latency measures, TetTox mice displayed signi ficantly higher avoidance success rates at 0.4 and 0.5 mA (Fig.4g), indicating that CGRP silencing expanded the range of aversive intensities over which active avoidance behaviour could be successfully learned and expressed."

Hanny Hayeon Lee 2026 (2)¶

Hanny Hayeon Lee, Eul Han & Yong Sang Jo. (2026). CGRP inhibition in the PBN shifts defensive strategy from freezing to active avoidance in a platform-based active avoidance paradigm. npj Science of Learning. https://doi.org/10.1038/s41539-026-00453-3

q3 · i? · causal · r2

Omnibus two-way ANOVA on retention test 3 success rate (Fig. 4g) reported a significant CGRP manipulation × Footshock Intensity interaction, F(4,48) = 13.65, ***p < 0.001, with Bonferroni post-hoc group differences at 0.4 and 0.5 mA.

"Two-way ANOVA: signi ficant main effects of CGRP manipulation [F(1,12) = 7.24, *p < 0.05], Footshock Intensity [F(4,48) = 10.53, ***p < 0.001], and their interaction [ F(4,48) = 13.65, ***p < 0.001]."

Discussion¶

Related Claims¶

• CGRP silencing reduces freezing at high footshock intensities where control mice show significant increases in freezing — related
• Low footshock intensities (0.1-0.2 mA) yield weak avoidance learning in both CGRP-silenced and control mice — related
• In the platform-based active avoidance paradigm, 0.3 mA footshock optimally supports cue-evoked active avoidance, with lower intensities yielding weak avoidance and higher intensities promoting freezing that suppresses action — related
• Freezing levels are negatively correlated with active avoidance performance across mice in the platform-based active avoidance paradigm — related