Auditory thalamic and cortical input to BLA interneurons is concentrated in the LA: thalamic EPSCs are larger in LA than BA interneurons (191 vs 53 pA, p < 0.0001)¶

Claim · All claims
Evidence · 1 study (2 entries) · causal r1–r2 · q3
Currency · newest study 2020 · not yet checked against its sources · not reviewed by a person

Subclaims¶

q3 i? Direct thalamic (AT) input reaches ~91% of LA interneurons but 67% of BA interneurons, and AT-evoked EPSCs are significantly smaller in BA (53 ± 14 pA) than LA (191 ± 18 pA; p < 0.0001). → Polepalli 2020 q3 i? Cortical (AC) input innervates 85% of LA but only 38% of BA interneurons; individual AC input amplitude does not differ between nuclei (p = 0.5888). → Polepalli 2020 (2)

Evidence¶

Polepalli 2020¶

Polepalli, J. S., Gooch, H. & Sah, P. (2020). Diversity of interneurons in the lateral and basal amygdala. npj Science of Learning 5:10. https://doi.org/10.1038/s41539-020-0071-z

q3 · i? · causal · r1

Optogenetic (ChR2) stimulation of AT afferents in acute slices from GAD67-GFP mice; BA AT-EPSC peak 53 ± 14 pA vs LA 191 ± 18 pA, p < 0.0001. "and it was significantly smaller (peak amplitude: 53 ± 14 pA; p < 0.0001)". No standardised effect size printed.

"In the BA, 67% (12/18) of interneurons received AT input (Fig. 3a) and it was significantly smaller (peak amplitude: 53 ± 14 pA; p < 0.0001) as compared to interneurons in the LA"

Polepalli 2020 (2)¶

Polepalli, J. S., Gooch, H. & Sah, P. (2020). Diversity of interneurons in the lateral and basal amygdala. npj Science of Learning 5:10. https://doi.org/10.1038/s41539-020-0071-z

q3 · i? · causal · r2

ChR2 stimulation of TeA cortical afferents in the same slice preparations; only 38% of BA interneurons (6/16) received AC input, with non-significant amplitude difference to LA (p = 0.5888). "input to individual cells (127 ± 50 pA) was not significantly different".

"AC input to BA interneurons was also sparse with 38% of cells (6/16; Fig. 3b) being innervated, but input to individual cells (127 ± 50 pA) was not significantly different to that in the LA ( p = 0.5888)"

Discussion¶

Related Claims¶

• Thalamic input to accommodating (ACC) LA interneurons is significantly larger than cortical input to the same subtype — related
• Auditory input drives feed-forward inhibition onto LA interneurons, with cortical input producing a smaller E/I ratio than thalamic input — related
• NMDAR subunit stoichiometry differs between LA and BA interneurons: LA NMDAR EPSCs decay faster and LA interneurons include a GluN2A-dominant majority plus NMDAR-lacking cells, absent in the BA — related
• Glutamatergic synapses on BA interneurons are less rectifying than those on LA interneurons, indicating fewer calcium-permeable GluR2-lacking AMPARs in the BA — related
• Long-term potentiation at cortical inputs is restricted to LA interneurons and cannot be induced in BA interneurons — related
• Experimental work indicates basolateral amygdala cells activated by appetitive CSs mainly encode behavioural output, not CS identity, challenging amygdalo-centric feedforward accounts — related