NMDAR subunit stoichiometry differs between LA and BA interneurons: LA NMDAR EPSCs decay faster and LA interneurons include a GluN2A-dominant majority plus NMDAR-lacking cells, absent in the BA¶

Claim · All claims
Evidence · 1 study (2 entries) · causal r2 · q3
Currency · newest study 2020 · not yet checked against its sources · not reviewed by a person

Subclaims¶

q3 i? NMDAR EPSC decay time constants are faster in LA (59 ± 3 ms, n = 54) than BA (91 ± 7 ms, n = 45; p = 0.001, K-S test), consistent with more GluN2A-containing receptors in LA. → Polepalli 2020 q3 i? In the LA, 18% of interneurons lack synaptic NMDARs and 72% express largely GluN2A NMDARs; in the BA, 51% express GluN2A and 49% GluN2B, with no NMDAR-lacking cells. → Polepalli 2020 (2)

Evidence¶

Polepalli 2020¶

Polepalli, J. S., Gooch, H. & Sah, P. (2020). Diversity of interneurons in the lateral and basal amygdala. npj Science of Learning 5:10. https://doi.org/10.1038/s41539-020-0071-z

q3 · i? · causal · r2

Weighted decay time constants measured at +40 mV with NBQX; "the NMDAR EPSC on interneurons in the LA had a faster decay time constant (59 ± 3 ms"; the article prints p = 0.001 (K-S test) but no standardised effect size.

"the NMDAR EPSC on interneurons in the LA had a faster decay time constant (59 ± 3 ms, n = 54) as compared to that in the BA (91 ± 7 ms, n = 45) (Fig. 5e inset; p = 0.001, K-S test)"

Polepalli 2020 (2)¶

Polepalli, J. S., Gooch, H. & Sah, P. (2020). Diversity of interneurons in the lateral and basal amygdala. npj Science of Learning 5:10. https://doi.org/10.1038/s41539-020-0071-z

q3 · i? · causal · r2

Proportions classified by NMDAR EPSC kinetics (τ < 80 ms GluN1/2A; τ > 80 ms GluN1/2B; none): "18% of interneurons had synapses that did not express synaptic NMDARs (n = 11/64)". Descriptive percentages; no effect size printed.

"in the LA, 18% of interneurons had synapses that did not express synaptic NMDARs ( n = 11/64), 72% expressed NMDARs largely composed of GluN2A-heterodimers ( n = 47/64), and 10% expressed GluN2B-containing NMDARs ( n = 7/64)"

Discussion¶

Related Claims¶

• Auditory thalamic and cortical input to BLA interneurons is concentrated in the LA: thalamic EPSCs are larger in LA than BA interneurons (191 vs 53 pA, p < 0.0001) — related
• Glutamatergic synapses on BA interneurons are less rectifying than those on LA interneurons, indicating fewer calcium-permeable GluR2-lacking AMPARs in the BA — related
• Long-term potentiation at cortical inputs is restricted to LA interneurons and cannot be induced in BA interneurons — related
• Thalamic input to accommodating (ACC) LA interneurons is significantly larger than cortical input to the same subtype
• Auditory input drives feed-forward inhibition onto LA interneurons, with cortical input producing a smaller E/I ratio than thalamic input
• Experimental work indicates basolateral amygdala cells activated by appetitive CSs mainly encode behavioural output, not CS identity, challenging amygdalo-centric feedforward accounts — related